Determination of Some miRNA Expression Levels in Chronic Lymphoid Leukemia Patients

Abstract

Chronic lymphoid leukemia (CLL) is a type of cancer that occurs as a result of the accumulation of morphologically small, mature-looking lymphocytes. CLL is the most common type of leukemia in Western countries, where it accounts for 30% of all leukemias. Accounts for only 10% of all leukemias in Asian populations. MicroRNAs (miRNAs) are non-protein-coding  single-stranded RNA molecules approximately 18-25 nucleotides long, forming a class of endogenous small RNAs. Research has shown that microRNAs can function as oncogenes or tumor suppressors in CLL. Although the expression levels of microRNA 133a and microRNA 452 have been determined in many cancers, including lung, prostate and colon cancer, expression levels in CLL patients have not been determined. Therefore, in our study, the expression levels of miRNA 133a and miRNA 452 in CLL patients will be calculated quantitatively using the Real-Time PCR method. As for the method steps, in the first stage, whole blood samples will be taken. miRNA will be isolated from the whole blood samples taken, cDNA will be synthesized from the miRNA samples, and finally, expression levels will be determined with the Real-Time PCR method using miRNA 133a, miRNA452 specific primers and U6 primer as the reference gene. The data obtained will be analyzed and interpreted with the SPSS package program. This study will be conducted to determine whether these two miRNAs can guide early diagnosis and diagnosis in CLL patients and to provide preliminary information to clinicians and contribute to the literature on this subject.