A Multiplex PCR Assay for Identification of Major Mastitis Causing Pathogens in Buffalo’s Raw Milk and Evaluation of Their Sensitivity

48 36

Authors

  • Muhammad Safdar Cholistan University of Veterinary and Animal Sciences
  • Yasmeen Junejo Cholistan University of Veterinary and Animal Sciences
  • Mehmet Ozaslan Gaziantep University

Abstract

Mastitis is a prevalent issue in dairy herds, particularly among buffaloes, leading to significant economic losses. This study developed a sensitive, specific, and cost-effective multiplex PCR assay for the identification of major pathogens responsible for mastitis in raw buffalo milk. A total of 200 milk samples were collected from various areas of District Multan and tested for the presence of pathogens. Additionally, the antibiotic susceptibility of drug-resistant bacteria was evaluated. The multiplex PCR assay demonstrated 100% specificity and high sensitivity (0.01ng/ul detection limit), identifying multiple pathogens simultaneously.

Downloads

Published

2024-09-21

How to Cite

Safdar, M., Junejo, Y., & Ozaslan, M. (2024). A Multiplex PCR Assay for Identification of Major Mastitis Causing Pathogens in Buffalo’s Raw Milk and Evaluation of Their Sensitivity. The Eurasia Proceedings of Health, Environment and Life Sciences, 1–5. Retrieved from https://ephels.net/index.php/ephels/article/view/123

Issue

VOLUME 14 (2024)

Section

Articles

License

Copyright (c) 2024 The Eurasia Proceedings of Health, Environment and Life Sciences

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

The articles may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Authors alone are responsible for the contents of their articles. The journal owns the copyright of the articles. The publisher shall not be liable for any loss, actions, claims, proceedings, demand, or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of the research material. All authors are requested to disclose any actual or potential conflict of interest including any financial, personal or other relationships with other people or organizations regarding the submitted work.