A Multiplex PCR Assay for Identification of Major Mastitis Causing Pathogens in Buffalo’s Raw Milk and Evaluation of Their Sensitivity

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Authors

  • Muhammad Safdar Cholistan University of Veterinary and Animal Sciences
  • Yasmeen Junejo Cholistan University of Veterinary and Animal Sciences
  • Mehmet Ozaslan Gaziantep University

DOI:

https://doi.org/10.55549/ephels.123

Abstract

Mastitis is a prevalent issue in dairy herds, particularly among buffaloes, leading to significant economic losses. This study developed a sensitive, specific, and cost-effective multiplex PCR assay for the identification of major pathogens responsible for mastitis in raw buffalo milk. A total of 200 milk samples were collected from various areas of District Multan and tested for the presence of pathogens. Additionally, the antibiotic susceptibility of drug-resistant bacteria was evaluated. The multiplex PCR assay demonstrated 100% specificity and high sensitivity (0.01ng/ul detection limit), identifying multiple pathogens simultaneously.

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Published

2024-09-01

How to Cite

Safdar, M., Junejo, Y., & Ozaslan, M. (2024). A Multiplex PCR Assay for Identification of Major Mastitis Causing Pathogens in Buffalo’s Raw Milk and Evaluation of Their Sensitivity. The Eurasia Proceedings of Health, Environment and Life Sciences, 14, 1–5. https://doi.org/10.55549/ephels.123

Issue

Vol. 14 (2024)

Section

Articles

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Copyright (c) 2024 The Eurasia Proceedings of Health, Environment and Life Sciences

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